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- Publisher
- Wiley
- Copyright
- Copyright © 1995 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1742-464X
- eISSN
- 1742-4658
- DOI
- 10.1111/j.1432-1033.1995.798zz.x
- Publisher site
- See Article on Publisher Site
Abstract
The similarity between oleate and linoleate desaturase sequences from several plants was used lo construct degenerate oligonucleotide primers for PCR experiments with cDNA transcribed from mRNA of ripening sunflower embryos. A DNA fragment was amplified and sequenced. Specific primers derived from this partial sequence were used for rapid amplification of the 3′‐ and 5′‐ends of this cDNA. With appropriate primers derived from these sequences, a full‐length clone of 1377 bp was amplified by PCR which, after sequencing, showed an open reading frame of 458 amino acids corresponding to a putative protein of about 52 kDa.
Journal
The Febs Journal – Wiley
Published: Sep 1, 1995
Keywords: ; ; ; ;