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Analysis of Macromolecular Polydispersity in Intensity Correlation Spectroscopy: The Method of CumulantsJournal of Chemical Physics, 57
- Publisher
- Wiley
- Copyright
- Copyright © 1989 Wiley Subscription Services
- ISSN
- 0022-2720
- eISSN
- 1365-2818
- DOI
- 10.1111/j.1365-2818.1989.tb02882.x
- Publisher site
- See Article on Publisher Site
Abstract
The method of cumulants has been applied to digital video fluorescence microscopy. The method is used to reconstruct the distribution of fluorescent molecules before the initiation of fluorescence photobleaching, and to characterize heterogeneous photobleaching by imaging one or more of the cumulants of the bleaching decay rate. Using the pipelined pixel processor of the image analysis system for the bulk of the calculations, rather than the general‐purpose host‐computer CPU, the video kinetics imaging can be performed in near real‐time. The method is applied to chick embryo myotubes labelled with fluorescein‐conjugated α‐bungarotoxin. The pre‐bleach fluorescence distribution is derived, and the image of fluorescein fluorescence is separated from glutaraldehyde‐induced autofluorescence on the basis of the spatially resolved average photobleaching decay rate.
Journal
Journal of Microscopy – Wiley
Published: Jan 1, 1989
Keywords: ; ; ; ; ; ; ;