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The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants

The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake,... • A bZIP transcription factor from Brassica juncea (BjCdR15) was isolated by the cDNA‐amplified fragment length polymorphism technique after cadmium treatment. Sequence analysis indicated high similarity between BjCdR15 and Arabidopsis TGA3. In Arabidopsis, TGA3 transcription is also induced by cadmium; hence, we investigated whether BjCdR15 is involved in cadmium tolerance and whether it can functionally replace TGA3 protein in Arabidopsis tga3‐2 mutant plants. • BjCdR15 expression was detected mainly in the epidermis and vascular system of cadmium‐treated plants, and increased in roots and leaves after cadmium treatment. The overexpression of BjCdR15 in Arabidopsis and tobacco enhanced cadmium tolerance: overexpressing plants showed high cadmium accumulation in shoots. Conversely, Arabidopsis tga3‐2 mutant plants showed high cadmium content in roots and inhibition of its transport to the shoot. • We demonstrated that BjCdR15 can functionally replace TGA3: in 35S::BjCdR15‐tga3‐2 plants, the long‐distance transport of cadmium from root to shoot was restored and these plants showed an increased cadmium content in shoots compared with all other assays. In addition, BjCdR15/TGA3 regulated the synthesis of phytochelatin synthase and the expression of several metal transporters. • The results indicate that BjCdR15/TGA3 transcription factors play a crucial role in the regulation of cadmium uptake by roots and in its long‐distance root to shoot transport. BjCdR15/TGA3 may thus be considered as useful candidates for potential biotechnological applications in the phytoextraction of cadmium from polluted soils. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png New Phytologist Wiley

The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants

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References (70)

Publisher
Wiley
Copyright
Copyright © 2010 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0028-646X
eISSN
1469-8137
DOI
10.1111/j.1469-8137.2009.03132.x
pmid
20028476
Publisher site
See Article on Publisher Site

Abstract

• A bZIP transcription factor from Brassica juncea (BjCdR15) was isolated by the cDNA‐amplified fragment length polymorphism technique after cadmium treatment. Sequence analysis indicated high similarity between BjCdR15 and Arabidopsis TGA3. In Arabidopsis, TGA3 transcription is also induced by cadmium; hence, we investigated whether BjCdR15 is involved in cadmium tolerance and whether it can functionally replace TGA3 protein in Arabidopsis tga3‐2 mutant plants. • BjCdR15 expression was detected mainly in the epidermis and vascular system of cadmium‐treated plants, and increased in roots and leaves after cadmium treatment. The overexpression of BjCdR15 in Arabidopsis and tobacco enhanced cadmium tolerance: overexpressing plants showed high cadmium accumulation in shoots. Conversely, Arabidopsis tga3‐2 mutant plants showed high cadmium content in roots and inhibition of its transport to the shoot. • We demonstrated that BjCdR15 can functionally replace TGA3: in 35S::BjCdR15‐tga3‐2 plants, the long‐distance transport of cadmium from root to shoot was restored and these plants showed an increased cadmium content in shoots compared with all other assays. In addition, BjCdR15/TGA3 regulated the synthesis of phytochelatin synthase and the expression of several metal transporters. • The results indicate that BjCdR15/TGA3 transcription factors play a crucial role in the regulation of cadmium uptake by roots and in its long‐distance root to shoot transport. BjCdR15/TGA3 may thus be considered as useful candidates for potential biotechnological applications in the phytoextraction of cadmium from polluted soils.

Journal

New Phytologist – Wiley

Published: Mar 1, 2010

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