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We present a method that offers the possibility to directly apply and measure torque on particles in an optical trap. It can be used to rotationally manipulate biopolymers attached to appropriate particles. A flat object is trapped and oriented in the focus of a linearly polarized laser light. The direction and power of the orientational trap are controlled by the polarization state of the light. As a demonstration of the capabilities of the method, we examined the torsional stiffness of dsDNA ( λ -DNA) in its linear torsional regime by directly measuring the torque generated by the molecule.
Physical Review Letters – American Physical Society (APS)
Published: Aug 4, 2006
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