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Direct Measurement of Torque in an Optical Trap and Its Application to Double-Strand DNA

Direct Measurement of Torque in an Optical Trap and Its Application to Double-Strand DNA We present a method that offers the possibility to directly apply and measure torque on particles in an optical trap. It can be used to rotationally manipulate biopolymers attached to appropriate particles. A flat object is trapped and oriented in the focus of a linearly polarized laser light. The direction and power of the orientational trap are controlled by the polarization state of the light. As a demonstration of the capabilities of the method, we examined the torsional stiffness of dsDNA ( λ -DNA) in its linear torsional regime by directly measuring the torque generated by the molecule. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Physical Review Letters American Physical Society (APS)

Direct Measurement of Torque in an Optical Trap and Its Application to Double-Strand DNA

4 pages

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References (30)

Publisher
American Physical Society (APS)
Copyright
Copyright © 2006 The American Physical Society
ISSN
1079-7114
DOI
10.1103/PhysRevLett.97.058301
pmid
17026144
Publisher site
See Article on Publisher Site

Abstract

We present a method that offers the possibility to directly apply and measure torque on particles in an optical trap. It can be used to rotationally manipulate biopolymers attached to appropriate particles. A flat object is trapped and oriented in the focus of a linearly polarized laser light. The direction and power of the orientational trap are controlled by the polarization state of the light. As a demonstration of the capabilities of the method, we examined the torsional stiffness of dsDNA ( λ -DNA) in its linear torsional regime by directly measuring the torque generated by the molecule.

Journal

Physical Review LettersAmerican Physical Society (APS)

Published: Aug 4, 2006

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