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References (35)
-
A. Brünger, R. Peters, K. Schulten (1985)
Continuous fluorescence microphotolysis to observe lateral diffusion in membranes. Theoretical methods and applicationsJournal of Chemical Physics, 82
-
(1990)
A novel technique for the determination of verapamil diffusion coefficients through hydroxypropyl methylcellulose gels . 1 -
( 1 9 9 2 ~ ) The use of Lucifer yellow , bodipy , FITC , TRITC , RITC and Texas red for dual irnmuno - fluorescence visualised with a confocal laser scanning microscope . 1 -
J. Blonk, A. Don, H. Aalst, J. Birmingham (1993)
Fluorescence photobleaching recovery in the confocal scanning light microscopeJournal of Microscopy, 169
-
D. Speyer, Z. Blank, W. Brenner (1969)
Diffusion from gelsJournal of Chemical Education, 46
-
9 9 2~) The use of Lucifer yellow, bodipy, FITC, TRITC, RITC and Texas red for dual irnmunofluorescence visualised with a confocal laser scanning microscope -
S. Chi, H. Jun (1991)
Release rates of ketoprofen from poloxamer gels in a membraneless diffusion cell.Journal of pharmaceutical sciences, 80 3
-
G. Conrath, B. Leclerc, Françoise Falson-Rieg, J. Devissaguet, G. Couarraze (1989)
In situ determination of the diffusion coefficient of a solute in a gel system using a radiotracerJournal of Controlled Release, 9
-
I. McKay, D. Forman, R. White (1981)
A comparison of fluorescein isothiocyanate and lissamine rhodamine (RB 200) as labels for antibody in the fluorescent antibody technique.Immunology, 43 3
-
S. Hibberd, J. Adler, M. Cheema (1990)
THE RAPID AND AUTOMATED CALCULATION OF DIFFUSION COEFFICIENTS IN THIN FILMS, GELS AND TISSUESJournal of Pharmacy and Pharmacology, 42
-
(1989)
Measurements of diffusion rates in hydrogel matrices using one dimensional -
D. Axelrod, D. Koppel, J. Schlessinger, E. Elson, W. Webb (1976)
Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.Biophysical journal, 16 9
-
(1981)
Diffusion in polyacrylarnide gels -
M. Scholz, H. Sauer, H. Rihs, R. Peters (1989)
Laser Scanning Microscopy To Study Molecular Transport In Single Cells, 1028
-
Alan Muhr, John Blanshard (1982)
Diffusion in gelsPolymer, 23
-
J. Tolley, J. Rassing (1983)
A new ultrasonic velocity scanning technique giving concentration-distance profiles and diffusion coefficient of drugs in gelsInternational Journal of Pharmaceutics, 14
-
(1989)
Measurements of diffusion rates in hydrogel matrices using one dimensional ' 9 ~ NMR imaging . 1 -
A. Entwistle, M. Noble (1992)
The use of Lucifer yellow, bodipy, FITC, TRITC, RITC and Texas red for dual immunofluorescence visualized with a confocal scanning laser microscopeJournal of Microscopy, 168
-
P. Dew, J. Walsh (1981)
A Set of Library Routines for Solving Parabolic Equations in One Space VariableACM Trans. Math. Softw., 7
-
B. Henry, J. Adler, S. Hibberd, M. Cheema, S. Davis, T. Rogers (1992)
Epi‐fluorescence Microscopy and Image Analysis Used to Measure Diffusion Coefficients in Gel SystemsJournal of Pharmacy and Pharmacology, 44
-
Shwu-Eng Webb, Fred Kuchler (1913)
A ComparisonTexas Medical Journal, 29
-
(1974)
A micro - fluorimetric study of translational diiusion in erythrocyte membranes -
G. Johnson, R. Davidson, K. McNamee, G. Russell, D. Goodwin, E. Holborow (1982)
Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy.Journal of immunological methods, 55 2
-
R. Peters, R. Peters, Jutta Peters, Jutta Peters, Karl-Heinz Tews, Karl-Heinz Tews, W. Bähr, Wolfgang Bähr (1974)
A microfluorimetric study of translational diffusion in erythrocyte membranes.Biochimica et biophysica acta, 367 3
-
(1981)
Diffusion in polyacrylarnide -
P. Wedekind, Ulrich Kubitscheck, Reiner Peters (1994)
Scanning microphotolysis: A new photobleaching technique based on fast intensity modulation of a scanned laser beam and confocal imagingJournal of Microscopy, 176
-
J. Wesson, H. Takezoe, Hyuk Yu, Stephen Chen (1982)
Dye diffusion in swollen gels by forced Rayleigh scatteringJournal of Applied Physics, 53
-
A. Axelsson, Bengt Westrin, D. Loyd (1991)
Application of the diffussion cell for the measurement of diffusion in gelsChemical Engineering Science, 46
-
W. Brown, R. Johnsen (1981)
Diffusion in polyacrylamide gelsPolymer, 22
-
(1989)
Analysis of heterogenous fluorescence photobleaching by video kinetics imaging : the method of cumulants . 1 -
Reiner Peters, Axel Brunger, Klaus Schulten (1981)
Continuous fluorescence microphotolysis: A sensitive method for study of diffusion processes in single cells.Proceedings of the National Academy of Sciences of the United States of America, 78 2
-
(1991)
Application of the diffusion cell for the measurement of diiusion in gels -
D. Koppel, Curt Carlson, H. Smilowitz (1989)
Analysis of heterogeneous fluorescence photobleaching by video kinetics imaging: The method of cumulantsJournal of Microscopy, 155
-
D. Koppel (1979)
Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics.Biophysical journal, 28 2
-
(1989)
Measurements of diffusion rates in hydrogel matrices using one dimensional ' 9~ NMR imaging
- Publisher
- Wiley
- Copyright
- Copyright © 1995 Wiley Subscription Services
- ISSN
- 0022-2720
- eISSN
- 1365-2818
- DOI
- 10.1111/j.1365-2818.1995.tb03668.x
- Publisher site
- See Article on Publisher Site
Abstract
The development of a photobleaching technique, CFMM (continuous fluorescence multipoint microphotolysis), to measur e diffusion coefficients in gel systems using a confocal scanning laser microscope is described. Diffusion coefficients (D) were determined for fluorescently labelled dextrans of varying molecular weight in agarose gels, and the results compared with two other methods. CFMM enabled diffusion coefficients to be rapidly determined from the profile across an irradiated area within a defined microscopic location of the gel. The technique was experimentally simple and produced values of D that corresponded well with classical double‐diffusion cell methods.
Journal
Journal of Microscopy – Wiley
Published: Jan 1, 1995
Keywords: ; ; ; ; ; ; ; ; ; ;