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Differential expression of four connexin genes, Cx-26, Cx-30.3, Cx-32, and Cx-43, in the porcine ovarian follicle

Differential expression of four connexin genes, Cx-26, Cx-30.3, Cx-32, and Cx-43, in the porcine... Abstract Connexin genes expressed in porcine ovaries were isolated by reverse transcription-PCR, and the expression of four connexin genes (Cx-43, -32, -30.3, and -26) was detected by in situ hybridization in internal and surrounding compartments of large antral follicles. Cx-43 and Cx-30.3 were expressed in the theca interna as well as in the granulosa cell compartment. However, two zones were observed in the theca interna by probing with Cx-43; about a quarter of the region close to the lamina basalis appeared devoid of Cx-43 messenger RNA (mRNA) and protein as well. Cx-30.3, in contrast, seemed to be expressed ubiquitously in these two compartments. Cx-26 and Cx-32 were expressed only in the thecal cells. Both Cx-43 and -30.3 also gave positive signals in the cumulus cells to the same extent as obtained in the granulosa cells with the respective probes, whereas the expression of Cx-32 and -26 was undetectable. Cx-43 mRNA drastically decreased in the functioning stage of the corpus luteum, whereas Cx-30.3 mRNA increased significantly in the early stage of luteinization in the estrus cycles. These results suggest that Cx-43 is not the only gap junction protein to be expressed in the ovarian follicle; other proteins, such as Cx-32, -30.3, and -26 seem to be expressed, and their expressions are regulated differently. This content is only available as a PDF. Copyright © 1996 by The Endocrine Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Endocrinology Oxford University Press

Differential expression of four connexin genes, Cx-26, Cx-30.3, Cx-32, and Cx-43, in the porcine ovarian follicle

Itahana,, K; Morikazu,, Y; Takeya,, T
Endocrinology , Volume 137 (11) – Nov 1, 1996
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References (32)

Publisher
Oxford University Press
Copyright
Copyright © 1996 by The Endocrine Society
ISSN
0013-7227
eISSN
1945-7170
DOI
10.1210/endo.137.11.8895378
Publisher site
See Article on Publisher Site

Abstract

Abstract Connexin genes expressed in porcine ovaries were isolated by reverse transcription-PCR, and the expression of four connexin genes (Cx-43, -32, -30.3, and -26) was detected by in situ hybridization in internal and surrounding compartments of large antral follicles. Cx-43 and Cx-30.3 were expressed in the theca interna as well as in the granulosa cell compartment. However, two zones were observed in the theca interna by probing with Cx-43; about a quarter of the region close to the lamina basalis appeared devoid of Cx-43 messenger RNA (mRNA) and protein as well. Cx-30.3, in contrast, seemed to be expressed ubiquitously in these two compartments. Cx-26 and Cx-32 were expressed only in the thecal cells. Both Cx-43 and -30.3 also gave positive signals in the cumulus cells to the same extent as obtained in the granulosa cells with the respective probes, whereas the expression of Cx-32 and -26 was undetectable. Cx-43 mRNA drastically decreased in the functioning stage of the corpus luteum, whereas Cx-30.3 mRNA increased significantly in the early stage of luteinization in the estrus cycles. These results suggest that Cx-43 is not the only gap junction protein to be expressed in the ovarian follicle; other proteins, such as Cx-32, -30.3, and -26 seem to be expressed, and their expressions are regulated differently. This content is only available as a PDF. Copyright © 1996 by The Endocrine Society

Journal

Endocrinology – Oxford University Press

Published: Nov 1, 1996

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